Eight months ago: "Well, actually, I'd like to toss it."
Two weeks ago: "Okay, let's toss it."
One week ago: "Waaiiit... There's hope!!"
The state of our project was not the most promising one when I started. After three months we reached the state that we had re-invented the wheel successfully. Or at least it felt like. Two weeks ago we were close to finishing up and mentioning everything we did in our theses, but please let's don't write a paper and get as fast as possible to the next project.
BUT then we talked to some people who are experts in image processing and who have the same problem as we have it right now. Most people either ignored it or changed the project in a way that the did not have to care about it. The point is, that many people reconstruct shapes from fluorescent confocal images, but often not the absolute intensity value, i.e. the concentration of a certain protein at some depth in the sample.
We try to do exactly this and we ran into some issues. But now we have a simulation which will help us to predict how certain signals in the sample are changed (e.g. attenuated) by the sample and it looks like we will be able to quantify the concentration of our protein :-)
